Tumour-associated macrophages (IL-23+CD14+ subset) levels during bladder cancer progression

Document Type : Original Article

Authors

1 Chemistry(Biochemistry department), Suez University, Suez, Egypt

2 Professor of urology, Mmansoura university

3 Professor of Medical Biochemistry National Research Center, Giza, Egypt

4 Genetics Unit, Children Hospital, Mansoura University, Egypt

5 Suez University

Abstract

Background: Multiple studies have reported that tumour-associated-macrophages (TAMs), which are abundant in the tumour stroma, are related to poor prognosis in different tumours, such as pancreatic adenocarcinoma, gastric cancer, and bladder cancer (BC). They exert a pivotal role in modulating tumor-progression and adjusting response to immunotherapy. In this article, we strive to evaluate the significance of CD14-positive TAMs levels in relation to BC development. Methodology: Based on the immuno-phenotypic analysis, the counts of TAMs bearing CD14 and IL23-receptor (IL23R) fractions were determined in 15 healthy-controls and 26 BC-patients BC, using a panel of phycoerythrin (PE)-labelled monoclonal-antibodies. Concomitantly, serum-levels of IL23 and IL17 cytokines were identified by ELISA-technique. Results: Findings revealed a higher levels of CD14+ IL23+ TAMs in BC patients sera [P= 0.035] compared to controls, and this increment was positively associated with advancing in tumour-grade [P=0.001] and stage [P< 0.043]. In context of bladder carcinogenesis, concentration of the aforesaid TAM subpopulation correlates positively with increased levels of both IL23 [r = 0.66, P<0.01] and IL17 [r = 0.657, P<0.01]. Conclusions: The study suggested that CD14-positive TAMs, especially those harboring IL23R engage in inflammation-related tumourgenesis by fostering the up-regulation of the inflammatory IL23/17 immune axis, and further can be utilized as a prognostic marker for BC development and progression.

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